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Question about imaging lichen slide preps with a compound scope

  • 29 Jan 2023 2:09 PM
    Message # 13077458

    Hi all,


    I am hoping to get advice from the group about getting the best possible images of spores (e.g. from crustose lichens) and similar structures from my current microscopy setup.  Although I have a decent compound scope, the limited depth of field is giving me headaches in terms of getting good spore or ascus images - I'd like to do better than the attached images which I took recently.


    My current equipment:  Olympus CX-31 scope with Plan C N objectives.  Olympus TG-6 point-and-shoot camera, which I aim through the inserted ocular.


    I have long used the TG-series Olympus cameras with my Leica dissecting scope and get reasonable images that way.


    I do NOT see a DSLR in my future and a trinocular compound scope is years away, if ever in my budget. 


    My main question is:  How can I best get Z-stacks (I assume that might be necessary) of spores or other tiny lichen structures with this setup?  Anyone deal with this problem and have similar equipment?  Or is it a lost cause? :)

    2 files
  • 30 Jan 2023 7:53 AM
    Reply # 13078002 on 13077458
    Bruce McCune (Administrator)

    You should be able to get decent spore photos without stacking (which is, I would guess, almost impossible with hand held camera). I can see the spores fine in the closer version you sent, but I would suggest trying to get the spores against an empty background -- it often helps to gently squash the mount. Then take a bunch of photos and use photoshop (or equivalent) to select the best and arrange them on a single canvas. It helps to set the selection tool to have a diffuse edge (e.g. 10 pixels) so that there aren't cut/paste edges all over. After that it almost always helps to adjust the brightness/contrast to that the background is nearly white. People often skip this step with the result that both spores and background look unnaturally gray.


    Apothecial sections are harder because they often don't lie in one plane, are often too thick, and your camera autofocus will often be trying to focus on something you're not interested in, like air bubbles or dirt particles. I don't have a good remedy for those things apart from rigging up something to hold your camera in position (I think there are some cheap commercial devices for this -- maybe someone else knows) and using manual focusing.

    Last modified: 30 Jan 2023 7:58 AM | Bruce McCune (Administrator)
  • 30 Jan 2023 9:22 AM
    Reply # 13078112 on 13077458

    Thanks so much for your comments and advice, Bruce, I really appreciate it.

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