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Another northern California seaside crust - small-spored Aspicilia

  • 20 Sep 2025 12:38 PM
    Message # 13544162

    Hi everyone,


    I hope it's OK to post these crusts for ID help - let me know if this is too many! :)  This Aspicilia-like crust was found on a seaside boulder recently in northern CA and collected with a permit as part of a CALS bioblitz.  The combination of small spores, conidia length and K plus Y without norstictic acid crystal formation is not getting me anywhere in the several keys I tried.  I hope to eventually get a DNA barcode on the sample, though not sure how helpful that is in Aspicilia.  Any ideas would be appreciated!  I am pasting my iNat description below:


    Collected with permit as part of CALS bioblitz. A white areolate crust growing on boulder near the ocean, the areoles are about 0.5 mm in diameter and many contain small, dark sunken apothecia.


    Cross-sections through the apothecia show a hyaline hymenium and hypothecium and a dark green epihymenium. The hymenium measures about 120 microns in height. Several apos were sectioned and unfortunately most asci were empty or contained degenerating spores, but a few "normal-appearing", simple, hyaline spores were seen that measured 10-12 x 7 microns (n=4). Hard to be sure, but likely 8 spores/ascus. Under polarized light, there were large, chunky crystals in the thalline exciple mixed with the photobiont. The true exciple seemed unusually prominent and was colored pale brown, about 50 microns in width.


    Spot tests with K showed a yellow reaction under the dissecting scope and a strong but transient flush of bright yellow under the compound scope. No TLC done yet.


    A pycnidia cross-section was squashed and revealed numerous long, thin, curved conidia measuring about 17-20 microns in length.


    12 files
    Last modified: 20 Sep 2025 2:30 PM | Anonymous member
  • 20 Sep 2025 8:03 PM
    Reply # 13544216 on 13544162

    Hmmm... I am now realizing that most of the properties of this crust overlap with a collection I made of Lecanora gangaleoides the previous day, except the spores are a bit smaller and I did not sample a pycnidium from that Lecanora.  Could this be a thallus of that species where the apothecia are all eroded or incipient?  Uh oh.

  • 24 Sep 2025 8:05 AM
    Reply # 13545433 on 13544162
    Anonymous member (Administrator)

    Not sure about this. It has the habit of an Aspicilia, but the K+ yellow reaction not turning red would be unusual for the genus (unless norstictic acid was present in low concentration). Also the chunky crystals in the exciple are more like the Lecanora subfusca group (and fit with L. gangaleoides). I can't tell from the paraphyses photo if they are moniliform or not because the view of the tips is mostly from the top rather than the sides. I don't know what the spermatia of L. gangaleoides are like -- but probably this is in Brodo's monograph on the Lecanora subfusca group.

  • 25 Sep 2025 8:43 PM
    Reply # 13546079 on 13544162
    Thanks Bruce!  The conidia/spermatia are not described in Brodo's 1984 paper, but I see they are in the Sonoran Flora volume and described as: "

    filiform, (13-)14-15.5(-17) µm long", so perhaps similar although smaller than my specimen.


    My specimen will probably get an ITS barcode in the next few months and hopefully that will help with the ID.  I will also see if I can get a better view of the paraphyses soon.

  • 19 Jan 2026 3:22 PM
    Reply # 13587004 on 13544162

    Follow up from the ITS barcode - this is a nearly 100% match to a barcode of my own specimen of Lecanora gangaleoides collected nearby during the same blitz - that one is at:


    https://www.inaturalist.org/observations/312070352


    So this is presumably an eroded/degenerating specimen of that species, I think... 

  • 20 Jan 2026 4:51 PM
    Reply # 13587510 on 13544162
    Anonymous member (Administrator)

    Cool! Mystery solved!

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